Development and Validation of New Chromatographic Method for the Determination of Enantiomeric and Diastereomeric Purity of Solifenacin Succinate: An Antimuscarinic Agent

<table class="table-group" width="800px"><tr class="fig-image" id="a"><td><img alt="243491.fig.005a" src="https://static.hindawi.com/articles/cri/volume-2011/243491/figures/243491.fig.005a.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(a) </b>Unspike test sample</td></tr></table></td></tr><tr class="fig-image" id="b"><td><img alt="243491.fig.005b" src="https://static.hindawi.com/articles/cri/volume-2011/243491/figures/243491.fig.005b.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(b) </b>Spike test sample</td></tr></table></td></tr></table>

<table>Typical chromatogram of solifenacin test sample, (a) unspiked and (b) spiked with stereoisomers at 0.15% level.(Chromatographic conditions- Chiralpak-IC, 250 mm <svg height="8.7875004" id="M60" style="vertical-align:-0.3135pt" version="1.1" viewbox="0 0 10.125 8.7875004" width="10.125" xmlns="http://www.w3.org/2000/svg">
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</svg> 4.6 mm ID column; mobile phase- n-hexane : ethanol : isopropyl alcohol : diethyl amine (60 : 15 : 25 : 0.1, v/v/v/v); flow rate 1.0 mL/min; column temperature <svg height="11.0375" id="M61" style="vertical-align:-0.17555pt" version="1.1" viewbox="0 0 20.200001 11.0375" width="20.200001" xmlns="http://www.w3.org/2000/svg">
<g transform="matrix(1.25,0,0,-1.25,0,11.0375)">
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<tspan style="font-size: 12.50px; " x="0" y="0">3</tspan>
<tspan style="font-size: 12.50px; " x="6.2515001" y="0">0</tspan>
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</svg>C; wavelength 220 nm).</table>

Chromatography Research International

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Figure 5

Figure 5: Development and Validation of New Chromatographic Method for the Determination of Enantiomeric and Diastereomeric Purity of Solifenacin Succinate: An Antimuscarinic Agent 

Figure 5 | Development and Validation of New Chromatographic Method for the Determination of Enantiomeric and Diastereomeric Purity of Solifenacin Succinate: An Antimuscarinic Agent 