Table of Contents
Chromatography Research International
Volume 2011, Article ID 252543, 9 pages
Research Article

Free Energy Contribution to Gas Chromatographic Separation of Petroselinate and Oleate Esters

1Biochemical Technology Division, School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, 49 Tientalay 25 Road. Takham, Bangkhuntien, Bangkok 10150, Thailand
2Department of Nutrition, Faculty of Public Health, Mahidol University, Rachathewi, Bangkok 10400, Thailand

Received 16 July 2010; Revised 23 September 2010; Accepted 26 October 2010

Academic Editor: Alberto Chisvert

Copyright © 2011 Chanida Sansa-ard et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The ease of separation by gas chromatography between petroselinic and oleic acids depends on the alcohol moieties of their esters. The esters of higher molecular weight alcohols tend to be better separated on a 90%-biscyanopropyl-10%-cyanopropylphenyl polysiloxane capillary column (30 m × 0.25 mm i.d.). By analysis of free energies contribution from different parts of the molecules, it is tentatively concluded that the interaction between the double bond and the column stationary phase is interfered by the bulky alkyl group, and it is the major driving force for the separation of the two fatty acids.