Validated Stability Indicating HPTLC Method for the Determination of Dutasteride in Pharmaceutical Dosage Forms

Table 2

Summary of degradation products of DUTA under different stress conditions.

Stress conditions	𝑅𝑓 values of degradation products	Peak purity data 𝑟(𝑆,𝑀), 𝑟(𝑀,𝐸)
Acid hydrolysis, 0.1 N HCl, refluxed for 8 h at 50°C	0.55	0.9991, 0.9996
Alkaline hydrolysis, 1 N NaOH, refluxed for 4 h at 50°C	0.26, 0.55	0.9998, 0.9994
Oxidation (H2O2, 30% v/v), refluxed for 3 h at 50°C	0.39, 0.55	0.9996, 0.9995
Wet heat, refluxed for 10 h at 100°C	0.55	0.9993, 0.9996
Dry heat, heated in oven at 100°C for 24 h	Not detected	0.9990, 0.9994
Day light, exposed to sunlight for 48 h	Not detected	0.9993, 0.9996
UV light, exposed to UV light (254 nm) for 48 h	Not detected	0.9995, 0.9997