Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms

Figure 2

Typical chromatogram of (a) vinpocetine standard preparation and (b) vinpocetine test sample. (Chromatographic conditions-Zorbax C₁₈, 150 mm length × 4.6 mm ID with 5 μm particle size column; mobile phase-buffer (containing 1.54% w/v ammonium acetate solution): acetonitrile (40 : 60, v/v); flow rate 1.0 mLmin⁻¹; column temperature 30°C; wavelength 280 nm and injected volume 10 μL).

(a) Chromatogram of vinpocetine standard preparation

(b) Chromatogram of vinpocetine test sample