Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms

<table class="table-group" width="800px"><tr class="fig-image" id="a"><td><img alt="801656.fig.003a" src="https://static.hindawi.com/articles/cri/volume-2011/801656/figures/801656.fig.003a.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(a) </b>Chromatogram of blank</td></tr></table></td></tr><tr class="fig-image" id="b"><td><img alt="801656.fig.003b" src="https://static.hindawi.com/articles/cri/volume-2011/801656/figures/801656.fig.003b.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(b) </b>Chromatogram of LOD of vinpocetine</td></tr></table></td></tr><tr class="fig-image" id="c"><td><img alt="801656.fig.003c" src="https://static.hindawi.com/articles/cri/volume-2011/801656/figures/801656.fig.003c.jpg"/></td></tr><tr class="fig-caption"><td align="center"><table><tr><td><b>(c) </b>Chromatogram of LOQ of vinpocetine</td></tr></table></td></tr></table>

<table>Typical chromatogram of (a) blank, (b) LOD of vinpocetine, and (c) LOQ of vinpocetine. (Chromatographic conditions-Zorbax C<sub>18</sub>, 150 mm length × 4.6 mm ID with 5 <i>μ</i>m particle size column; mobile phase-buffer (containing 1.54% w/v ammonium acetate solution): acetonitrile (40 : 60, v/v); flow rate 1.0 mLmin<sup>−1</sup>; column temperature 30°C; wavelength 280 nm and injected volume 10 <i>μ</i>L).</table>

Chromatography Research International

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Figure 3

Figure 3: Development and Validation of RP-HPLC Method for Quantitative Estimation of Vinpocetine in Pure and Pharmaceutical Dosage Forms 