Table of Contents
Chromatography Research International
Volume 2012, Article ID 130732, 7 pages
http://dx.doi.org/10.1155/2012/130732
Review Article

Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies

1Faculty of Pharmaceutical Science, Kyushu University, 3-1-1 Maidashi, Higashiku, Fukuoka 812-0855, Japan
2Faculty of Pharmaceutical Science, Khon Kaen University, Khon Kaen 40002, Thailand
3Faculty of Pharmaceutical Science, Nagasaki International University/2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298, Japan

Received 18 May 2011; Accepted 9 November 2011

Academic Editor: Irena Vovk

Copyright © 2012 Hiroyuki Tanaka et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We succeeded in developing the fingerprint of natural product by eastern blotting using monoclonal antibodies. After developing and separating them on a TLC plate, solasodine glycosides are oxidized by NaIO4 and reacted with a protein to give conjugates which are recognized with anti-solamargine monoclonal antibody (MAb). Anti-solamargine MAb having wide cross-reactivity can stain and detect all solasodine glycosides by fingerprint. Different sensitivity between solamargine and solasonine was observed. The detection limit was 1.6 ng of solasonine. The hydrolysed products of solamargine were determined by fingerprint of eastern blotting compared to their Rf values depending on the sugar number. Fingerprint by eastern blotting using anti-ginsenoside Rb1 MAb distinguished the formula containing ginseng prescribed in traditional Chinese medicine. By double-staining of ginsenosides it is possible to suggest that the staining color shows the pharmacological activity, such as the purple bands indicate ginsenosides having stimulation activity, and the blue color indicated compound like ginsenosides possessed the depression affect for the central nervous system (CNS), respectively.