Fingerprinting of Natural Product by Eastern Blotting Using Monoclonal Antibodies
Double-staining of eastern blotting for ginsenosides contained in various ginseng using anti-ginseenoside-Rb1 and anti-ginsenoside-Rg1 monoclonal antibodies. (a) TLC profile stained by sulfuric acid, (b) Eastern blotting by anti-ginsenoside-Rb1 and anti-ginseside-Rg1 monoclonal antibodies. I, II, III, IV, V, and VI indicated white ginseng, red ginseng, fibrous ginseng (Panax ginseng), Panax notoginseng, Panax quinquefolius, and Panax japonicus, respectively. Upper purple color spots and lower blue color spots were stained by anti-ginsenoside-Rg1 and anti-ginsenoside-Rb1 monoclonal antibodies, respectively.
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