Research Article
Ion Suppression Study for Tetracyclines in Feed
Table 1
Sample pretreatment methods tested for the cleanup of tetracyclines from animal feeds.
| | Method | | 1 | 2 | 3 | 4 |
| Extraction | 40 mL McIlvaine buffer-EDTA 0,1 M | 40 mL DCM McIlvaine buffer-EDTA 0,1 M (1 : 3) | 40 mL McIlvaine buffer-EDTA 0,1 M | 40 mL McIlvaine buffer-EDTA 0,1 M |
| SPE Loading pH | 4.2 | 4.2 | 2.5 | 10 |
| Cartridge | Oasis HLB (60 mg) | Oasis HLB (60 mg) | Oasis MCX (60 mg) | Oasis MAX (60 mg) |
| Wash (2 mL) | H2O | H2O | (1) FA 2% (v/v) | (1) NH3 | (2) MeOH | (2) MeOH |
| Elution (2 mL) | MeOH | MeOH | MeOH : NH3 (95 : 5) | MeOH : FA (95 : 5) |
| Final step | Evaporation with N2 and reconstitution with mobile phase | Evaporation with N2 and reconstitution with mobile phase | Dilution with 8 mL acetic acid 10% (v/v) | Dilution with 8 mL H2O (v/v) |
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