Table of Contents
Chromatography Research International
Volume 2012, Article ID 162302, 4 pages
http://dx.doi.org/10.1155/2012/162302
Research Article

A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora

1Medicinal Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O.-CIMAP, Lucknow 226015, India
2Analytical Chemistry Department, Central Institute of Medicinal and Aromatic Plants, P.O.-CIMAP, Lucknow 226015, India

Received 2 February 2012; Revised 22 February 2012; Accepted 22 February 2012

Academic Editor: Sibel A. Ozkan

Copyright © 2012 Harish C. Upadhyay et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A rapid, sensitive, and reproducible RP-HPLC method was developed for the determination of two constituents in Ammannia multiflora, namely, tetralone-4-O-β-D-glucopyranoside (1) and 4-hydroxy-α-tetralone (2). The samples were separated on a Spherisorb ODS2 column (250×4.6 mm, i.d., 10 μm) and binary elution of water and methanol (2 : 3) with flow rate of 0.8 mL/min at λ 254 nm. The LOD and LOQ were found 0.05 and 0.18 μg/mL for compound 1 and 0.06 and 0.18 μg/mL for compound 2, respectively. All calibration curves showed good linearity (r2>0.999) within test ranges for both the analytes. The RSD values for intra-and inter-day precisions were less than 1.1%. The successful application of the developed method on five different samples revealed an average 0.0206% and 0.7636% (w/w) of compounds 1 and 2, respectively in A. multiflora indicating that the developed LC assay method may be readily utilized as a quality control method for the plant.