A Validated LC Method for Separation and Determination of Tetralone-4-O-β-D-Glucopyranoside and 4-Hydroxy-α-Tetralone in Ammannia multiflora

Table 2

Column performance report for the determination of analytes 1 and 2 (𝑛=5).

Analyte	Retention	Retention	Tailing	Theoretical
	time (min)	factor	factor	plates
1
Mean	4.1±0.02	3.13	1.58±0.0152	2053±0.08
% RSD	0.48	0.31	0.96	0.003
2
Mean	5.2±0.04	4.25	1.28±0.0142	3214±0.07
% RSD	0.76	0.35	0.95	0.002