Table of Contents
Chromatography Research International
Volume 2012, Article ID 180103, 6 pages
Research Article

HPTLC Fingerprint Profile and Isolation of Marker Compound of Ruellia tuberosa

1Pharmacognosy Department, Pioneer Pharmacy Degree College, Ajwa-Nimeta Road, Sayajipura, Vadodara 390019, India
2Herbal Drug Technology Lab, Pharmacy Department, Faculty of Technology and Engineering, The M. S. University of Baroda, Vadodara 390 001, India

Received 14 June 2011; Revised 21 September 2011; Accepted 30 September 2011

Academic Editor: Monika Waksmundzka-Hajnos

Copyright © 2012 Daya L. Chothani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa. From the HPTLC fingerprint the florescent band (under 366 nm) at 𝑅 𝑓 : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and stem of R. tuberosa. So, the florescent band (under 366 nm) at 𝑅 𝑓 : 0.56 was isolated as marker compound RT-F2 from root of R. tuberosa. The marker compound RT-F2 was quantified by using HPTLC technique. The percentage (W/W) amount of RT-F2 was found to 40.0% and 44.6% in petroleum ether and ethyl acetate extract of R. tuberosa roots, respectively. Further study is suggested to characterization and biological nature of marker compound.