Table of Contents
Chromatography Research International
Volume 2012, Article ID 691509, 7 pages
http://dx.doi.org/10.1155/2012/691509
Research Article

CZE/PAD and HPLC-UV/PAD Profile of Flavonoids from Maytenus aquifolium and Maytenus ilicifolia “espinheira santa” Leaves Extracts

Instituto de Química de São Carlos, Universidade de São Paulo, Caixa Postal 780, 13560-970 São Carlos, SP, Brazil

Received 22 June 2011; Accepted 9 October 2011

Academic Editor: Irena Vovk

Copyright © 2012 Cristina A. Diagone et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This paper describes the application of HPLC and CZE to analyze flavonoids in the leaves of Maytenus ilicifolia and Maytenus aquifolium, which are species widely used in Brazilian folk medicine. The two species showed different flavonoid profiles, but acidic hydrolysis of the Maytenus extracts confirmed that all these compounds are quercetin or kaempferol derivatives. A comparison of the CZE and HPLC profiles of Maytenus extracts showed numerous flavonoid peaks using HPLC. However, the advantages of CZE such as analysis without requiring clean-up and less generation of chemical waste than with HPLC point to the potential of the CZE technique for the quality control (routine analysis) of “espinheira santa” phytopharmaceuticals.