Table of Contents
Chromatography Research International
Volume 2012 (2012), Article ID 846574, 5 pages
http://dx.doi.org/10.1155/2012/846574
Research Article

A Validated RP-HPLC Method for the Estimation of Pizotifen in Pharmaceutical Dosage Form

1Department of Chemistry, Krishna University, Machilipatnam 521001, India
2Department of Chemistry, GITAM University, Visakhapatnam 530045, India
3Departmentof Chemistry, NIMS University, Rajasthan 303121, India

Received 10 August 2011; Revised 6 September 2011; Accepted 7 September 2011

Academic Editor: Sibel A. Ozkan

Copyright © 2012 M. V. Basaveswara Rao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A simple, selective, linear, precise, and accurate RP-HPLC method was developed and validated for rapid assay of Pizotifen in pharmaceutical dosage form. Isocratic elution at a flow rate of 1.0 mL/min was employed on Chromosil C18 (250 mm × 4.6 mm, 5 μm) column at ambient temperature. The mobile phase consists of methanol : acetonitrile in the ratio of 10 : 90 v/v. The UV detection wavelength was 230 nm, and 20 μL sample was injected. The retention time for Pizotifen was 2.019 min. The percent RSD for accuracy of the method was found to be 0.2603%. The method was validated as per the ICH guidelines. The method can be successfully applied for routine analysis of Pizotifen in the rapid and reliable determination of Pizotifen in pharmaceutical dosage form.