A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

Table 2

Robustness study data obtained from HPLC.

Robustness conditions		RT (min)	ASSAY (%)	System suitability data
Type	Rate			Theoretical plate	Asymmetry
Flow rate	0.9 mL/min.	3.59	100.09	3498	0.97
	1.1 mL/min.	2.95	99.84	3607	1.03
Temperature	28°C	3.24	98.97	3930	1.07
	32°C	3.22	99.68	3890	1.50
Mobile phase	72 : 28	3.16	101.07	4027	1.67
	68 : 32	3.32	99.42	4145	1.09