A Comparative Validation Study of Fluconazole by HPLC and UPLC with Forced Degradation Study

Table 4

Comparison of HPLC and UPLC methods by validation results.

Validation parameter	HPLC method	UPLC method
Accuracy (% Recovery)	99-100	99-100
Linearity
Regression equation	𝑦=2907𝑥+6956	𝑦=89810𝑥+95164
Corelation coefficient (𝑅^2)	0.984	0.998
Precision
Intermediate precision	(% RSD) 0.61	(% RSD) 1.18
Method precision	(% RSD) 0.71	(% RSD) 0.70
Robustness	Highly robustness method	Highly robustness method
Specificity	No interference found to analyte peak	No interference found to analyte peak
Limit of detection	0.5 µg/mL	0.3 µg/mL
Limit of quantification	1.5 µg/mL	1.4 µg/mL
Total analysis time	7 minutes	3 minutes