Simultaneous Quantification of Glibenclamide, Simvastatin, and Quercetin by Using LC-UV Method and Its Application to Pharmacokinetic Study in Rats

Table 2

Stability data of glibenclamide, simvastatin, and quercetin in rat plasma.

QC samples	Mean concentration (ng mL^−1) observed at 0 h			Mean concentration (ng mL^−1) observed at last h			CV%
	Glibenclamide	Simvastatin	Quercetin	Glibenclamide	Simvastatin	Quercetin	Glibenclamide	Simvastatin	Quercetin
Bench top stability (𝑛=6) (after 6 h)
High	852.1	846.3	852.9	754.9	823.8	857.9	3.6	5.3	2.8
Low	46.4	48.1	45.3	45.3	46.6	48.4	6.2	4.7	3.7
Freeze thaw stability (𝑛=6) (three cycles)
High	859.2	857.2	847.9	856.4	848.4	861.2	3.7	6.2	1.8
Low	45.2	46.4	45.1	48.6	46.9	46.1	5.2	4.1	5.8
Dry extract stability (𝑛=6) (24 h)
High	869.3	862.8	872.2	853.6	855	857	2.5	2.7	3
Low	46	46.8	48.2	47.9	46.7	48.3	5.5	3.9	5.7
Auto sampler stability (𝑛=6) (12 h)
High	841.4	866.4	851.4	864.3	852.5	868.5	2.8	1.7	2.8
Low	46.8	45.3	47.6	47.7	46.7	47.5	6.6	2.9	3.7
Long- term stability (𝑛=6) (30 days)
High	842.3	847.8	869.4	864.4	847.5	854.8	1.8	3.6	2.9
Low	46.8	45.7	46.9	45.8	48.7	46.8	2.8	5.4	4.4

Data is expressed as mean and CV%.