Validated HPLC Method for Quantification of Pregabalin in Human Plasma Using 1-Fluoro-2,4-dinitrobenzene as Derivatization Agent
Typical HPLC chromatograms of pregabalin and I.S. derivative. (a) Drug free plasma; (b) plasma spiked with pregabalin (5 ng/mL) and I.S.; (c) plasma spiked with pregabalin (100 ng/mL) and I.S.; (d) a real sample (concentration of pregabalin was determined as 33 ng/mL).
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