Validated HPLC Method for Quantification of Pregabalin in Human Plasma Using 1-Fluoro-2,4-dinitrobenzene as Derivatization Agent

Table 3

A brief comparison between different HPLC methods for determination of pregabalin in human plasma.

Reference	Derivatizing reagent	Linearity range; (g/mL)	Detection	Retention time (min)	LOQ (μg/mL)
[15]	Picrylsulfonic acid	0.5–8	Fluorescence	17.0	0.25
[16]	o-Phthaldialdehyde	0–40	Fluorescence	15.0	0.13
[24]	4-Chloro-7-nitrobenzofuran	0.4–30	Fluorescence	14.0	0.4
The method developed in this study	1-Fluoro-2,4-dinitrobenzene	1–5000	UV	5.3	0.001