Chromatography Research International / 2014 / Article / Tab 3

Research Article

Validated HPLC Method for Quantification of Pregabalin in Human Plasma Using 1-Fluoro-2,4-dinitrobenzene as Derivatization Agent

Table 3

A brief comparison between different HPLC methods for determination of pregabalin in human plasma.

ReferenceDerivatizing reagentLinearity range
(g/mL)
DetectionRetention time (min)LOQ (μg/mL)

[15]Picrylsulfonic acid0.5–8 Fluorescence17.0 0.25
[16]o-Phthaldialdehyde0–40 Fluorescence15.0 0.13
[24]4-Chloro-7-nitrobenzofuran0.4–30 Fluorescence14.0 0.4
The method developed in this study1-Fluoro-2,4-dinitrobenzene1–5000 UV5.3 0.001

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