Table of Contents
Chromatography Research International
Volume 2014 (2014), Article ID 469562, 10 pages
Review Article

Determination of Strong Acidic Drugs in Biological Matrices: A Review of Separation Methods

1Medical College, Hunan Normal University, Changsha 410006, China
2School of Pharmaceutical Sciences, Central South University, Changsha 410013, China

Received 3 June 2014; Revised 17 September 2014; Accepted 17 September 2014; Published 29 September 2014

Academic Editor: Toyohide Takeuchi

Copyright © 2014 Lingli Mu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Strong acidic drugs are a class of chemical compounds that normally have high hydrophilicity and large negative charges, such as organophosphatic compounds and organosulphonic compounds. This review focuses on sample preparation and separation methods for this group of compounds in biological matrices in recent years. A wide range of separation techniques, especially chromatographic method, are presented and critically discussed, which include liquid chromatography (e.g., ion-pair and ion-exchange chromatography), capillary electrophoresis (CE), and other types. Due to the extremely low concentration level of target analytes as well as the complexity of biological matrices, sample pretreatment methods, such as dilute and shoot methods, protein precipitation (PP), liquid-liquid extraction (LLE), solid-phase extraction (SPE), degradation, and derivatization strategy, also play important roles for the development of successful analytical methods and thus are also discussed.