Table of Contents
Chromatography Research International
Volume 2014, Article ID 518175, 8 pages
Research Article

Identification and Quantification of Aldose Reductase Inhibitory Flavonoids in Herbal Formulation and Extract of Gymnema sylvestre Using HPLC-PDA and LC-MS/MS

Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Hyderabad, Balanagar, Hyderabad 500037, India

Received 11 August 2014; Revised 20 October 2014; Accepted 1 November 2014; Published 26 November 2014

Academic Editor: Branca Silva

Copyright © 2014 Nanjappan Satheeshkumar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Adulteration of herbal supplements is a major issue for many countries. A simple and reliable HPLC-PDA method was developed for quantification of aldose reductase inhibitory flavonoids rutin, quercetin, and kaempferol. The chromatographic separation was performed on a Fortis C18 column in gradient mode with detection at 267 nm. The presence of these markers was confirmed through the accurate m/z values and MS/MS data obtained using quadruple time of flight mass spectrometry (QTOF-MS). The proposed method was successfully applied to examine the amount of these active constituents in antiobese polyherbal formulation and plant extract of Gymnema sylvestre.