Stability Indicating Liquid Chromatographic Method for Estimation of Trihexyphenidyl Hydrochloride and Risperidone in Tablet Formulation: Development and Validation Consideration

Table 1

Summary of HPLC method development and validation.

Parameters	Data obtained
	RSP	THP
Theoretical plate	3508±230	2670±190
Retention time (min)	1.10±0.01	1.53±0.01
Correlation coefficient	0.998±0.01	0.997±0.01
Linearity (𝑛=6)	50–175 (𝜇g/mL)	50–175 (𝜇g/mL)
Precision (% RSD)
Intraday	0.047	0.368
Interday	0.273	0.561
Repeatability (𝑛=6)	0.181	0.656
Accuracy (% recovery)	99.42–100.26%	99.06–100.42%
LOD	0.40 (𝜇g/mL)	1.24 (𝜇g/mL)
LOQ	1.29 (𝜇g/mL)	3.92 (𝜇g/mL)