Development and Validation of Stability-Indicating RP-UPLC Method for Simultaneous Determination of Related Substances of S(−)Amlodipine and S(−)Metoprolol Succinate in Fixed Dose Combination Tablet Dosage Form

<table class="table-group" id="tab8"><tr><td><table class="table"><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr class="thead"><td align="left">Analyte</td><td align="center">Slope</td><td align="center">RRF</td></tr><tr><td class="thead-hr" colspan="3"><hr/></td></tr><tr><td align="left">S(−)Metoprolol</td><td align="center">18015</td><td align="center">1.0</td></tr><tr><td align="left">S(−)Amlodipine</td><td align="center">48003</td><td align="center">1.0</td></tr><tr><td align="left">Metoprolol Succinamide impurity</td><td align="center">20981</td><td align="center">1.16</td></tr><tr><td align="left">Amlodipine related compound-A</td><td align="center">31669</td><td align="center">0.66</td></tr><tr class="table-tr"><td colspan="3"><hr class="tbody-hr"/></td></tr></table></td></tr></table>

Chromatography Research International

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Table 8

Table 8: Development and Validation of Stability-Indicating RP-UPLC Method for Simultaneous Determination of Related Substances of S(−)Amlodipine and S(−)Metoprolol Succinate in Fixed Dose Combination Tablet Dosage Form 

Table 8 | Development and Validation of Stability-Indicating RP-UPLC Method for Simultaneous Determination of Related Substances of S(−)Amlodipine and S(−)Metoprolol Succinate in Fixed Dose Combination Tablet Dosage Form 