Research Article
A Simple Analytical Method for High-Throughput Screening of Major Sugars from Soybean by Normal-Phase HPLC with Evaporative Light Scattering Detection
Table 2
Method validation including detection limit, repeatability and spike recovery.
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Detection limit
ppm | Repeatability | Recovery (%) ± RSD | | Intraday (RSD%) | Interday (RSD%) | Spike to dried sample | Spike to sugar extract | | | | |
| | Fructose | 60.0 | 2.2 | 6.0 | 103 ± 1.5 | 101 ± 2.0 | | Galactose | 135.0 | — | — | 105 ± 3.2 | 111 ± 1.2 | | Glucose | 30.0 | 2.0 | 9.0 | 101 ± 1.1 | 99 ± 1.0 | | Sucrose | 9.3 | 1.2 | 2.9 | 99 ± 3.0 | 98 ± 4.4 | | Melibiose | 19.4 | 1.7 | 12.0 | 73 ± 1.4 | 92 ± 0.5 | | Raffinose | 12.1 | 1.7 | 2.9 | 102 ± 2.8 | 101 ± 1.6 | | Stachyose | 10.0 | 1.2 | 4.7 | 102 ± 0.9 | 102 ± 3.9 |
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