Chromatography Research International / 2016 / Article / Tab 6

Research Article

Validated Stability Indicating Chromatographic Method for the Simultaneous Estimation of Camylofin with NSAID Drugs and a New Approach of Method Transfer from Classical HPLC to a Modern UPLC Instrument

Table 6

Significant values in comparison with UPLC and HPLC.

Sr. no.Chromatographic conditionsInstrumentation
UPLCHPLC

1Flow (mL/min)0.2501.0
2Injection volume (L)1.020.0
3Amount loaded on column (g)0.051.00
4Run time (min)5.020.0

5Drugs CMFDCFCMFDCF

6Tailing factor ()1.4830.9561.331.34
7Resolution ()11.8725.90
8Retention time (min)1.13.22.613.7
9Theoretical plates ()1144329626787534

Significance of HPLC to UPLC method transfer.

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