Chromatography Research International / 2016 / Article / Tab 6

Research Article

Validated Stability Indicating Chromatographic Method for the Simultaneous Estimation of Camylofin with NSAID Drugs and a New Approach of Method Transfer from Classical HPLC to a Modern UPLC Instrument

Table 6

Significant values in comparison with UPLC and HPLC.

Sr. no.Chromatographic conditionsInstrumentation

1Flow (mL/min)0.2501.0
2Injection volume (L)1.020.0
3Amount loaded on column (g)0.051.00
4Run time (min)5.020.0


6Tailing factor ()1.4830.9561.331.34
7Resolution ()11.8725.90
8Retention time (min)
9Theoretical plates ()1144329626787534

Significance of HPLC to UPLC method transfer.

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