Development and Validation of RP-HPLC Method for the Determination of Hydrochlorothiazide in Bulk Drug and Pharmaceutical Dosage Form

Table 2

Effect of injection volume on peak area and retention time of HCTZ peak.

Injection volume (L)	(min)	Peak area
10	3.41	192007
20	3.36	384602
30	3.33	574737