A Histopathological and Immunohistochemical Analysis of Ameloblastic Fibrodentinoma

Table 1

The antibodies used to detect the expression of the different proteins in the epithelial and mesenchymal cells of the AFD samples.

Antibody	Source/clone	Dilution	Antigen retrieval	Epithelial cells	Mesenchymal cells
Amelogenin	Santa Cruz/SC-33109	1 : 100	Mw	+	Neg
CK19	Genetex/polyclonal	1 : 100	Mw	+++	Neg
Calretinin	DAKO/DAK-CALERET 1	1 : 100	Mw	+	Neg
E-cadherin	DAKO/NCH-38	1 : 100	Mw	++	Neg
Syndecan-1	DAKO/MI15	1 : 100	Mw	+++	++
Histone H3	GeneTex/E107	1 : 100	Mw	+++	+++
Ki-67	DAKO/MIB1	1 : 100	Mw	+	+
MSH-2	Genetex/EPR3943	1 : 100	Mw	+++	+++

Neg: negative, +: weak, ++: moderate, +++: strong immunoexpression, and Mw: microwave.