A Fatal Case of Primary Basaloid Squamous Cell Carcinoma in the Intrahepatic Bile Ducts

Table 2

Primer sequences (For., Rev., Seq.), amplified fragment sizes, and pyrosequencing dispensation and analysis orders (Disp. and Anal.) used for mutational analysis of KRAS, IDH1, and IDH2.

Exon		Primer sequence	Fragment (bp)	Hot spot codons
KRAS
2	For.	5′-GGCCTGCTGAAAATGACTG	79	12, 13
	Rev.	5′-Biotin-GCTGTATCGTCAAGGCACTCT
	Seq.	5′-CTTGTGGTAGTTGGAGCT
	Disp.	TACGACTCAGATCGTAG
	Anal.	GNTGRCGTAGGYA
3	For.	5′-CAG ACT GTG TTT CTC CCT TCT CA	133	61
	Rev.	5′-Biotin-TCCTCATGTACTGGTCCCTCATT
	Seq.	5′-TTG GAT ATT CTC GAC ACA
	Disp.	TCAGCAGATCGTAGAG
	Anal.	G/ACAGGTCNAGAGGAGAG
IDH1
4	For.	5′-Biotin-TGGATGGGTAAAACCTATCATCA	66	132
	Rev.	5′-TTGCCAACATGACTTACTTGATC
	Seq.	5′-ATCTGTGAATCCAGAGGGG
	Disp.	TGCACTGTCACTA
	Anal.	GAC/TG/AACCTAT
IDH2
4	For.	5′-TGTGGAAAAGTCCCAATGGA	176	132
	Rev.	5′-Biotin-AGGTCAGTGGATCCCCTCTC
	Seq.	5′-GCCCATCACCATTGG
	Disp.	GCATGCACG
	Anal.	CAG/A/TGCACGCC