Hemangioblastoma in the Lung: Metastatic or Primary Lesions?

Table 1

Results of immunohistochemistry studies on pulmonary hemangioblastoma.

Antibodies	Immunohistochemistry results	Clone/dilution/company
Inhibin	Stromal cell positive	R1/prediluted/VMS^c
NSE^a	Stromal cell positive	Polyclonal/1 : 100/Zymed
S-100	Stromal cell positive	Polyclonal/1 : 900/DACO
Vimentin	Stromal cell positive	V9/prediluted/VMS^c
Calretinin	Rare stromal cell positive	Polyclonal/1 : 25/Introgen
Pancytokeratin	Negative	AE1/3/PCK26/prediluted/VMS^c
EMA^b	Negative	E29/prediluted/Cell Marque
CD10	Negative	SP67/prediluted/VMS^c
Melan A	Negative	MART-1/prediluted/VMS^c
Synaptophysin	Negative	SP11/prediluted/VMS^c
Chromogranin	Negative	Polyclonal/1 : 1600/DACO
GFAP	Negative	Polyclonal/1 : 1600/DACO
CD68	Negative	KP1/1 : 3600/DACO
Lysozyme	Negative	Polyclonal/1 : 3600/DACO
CD1a	Negative	EP3622/prediluted/VMS^c
CD34	Endothelial cell positive	QBend10/prediluted/VMS^c
Ki-67	Estimated 5% of interstitial tumor cells positive	MIB1/1 : 25/DACO

NSE: neuron specific enolase
^bEMA: embryonic membrane antigen
^cVMS: Ventana Medical System Inc.
Note: CD34, lysozyme, calretinin, and NSE are done on small tumor nodule; the rest are done on larger tumor nodule.