Clonal CD8+ T Lymphocytic Proliferation and Karyotypical Abnormalities in an EBV Associated Hemophagocytic Lymphohistiocytosis

Figure 3

Molecular and cytogenetics findings in the bone marrow. (a) and (b) Assessment of TCR gamma receptor gene rearrangement by PCR on formalin-fixed, paraffin-embedded tissue sections from bone marrow clot demonstrated biclonal PCR product using Vγ 1-8,9/Jγ1/2 primer set (a) and monoclonal PCR product using Alt. Vγ/Jγ1/2 primer sets (b). (c) G-band karyotypic study on bone marrow aspirate demonstrated an abnormal karyotype in all 20 dividing cells examined: 49,XY,+5,+11,+20/.

(a)

(b)

(c)