A Biphasic Pleural Tumor with Features of an Epithelioid and Small Cell Mesothelioma: Morphologic and Molecular Findings

Table 1

Immunostaining results for large and small cell components of biphasic tumor.

Immunostain	Mesothelial areas	Small blue cell areas
MyoD1	Positive, diffuse	Positive, punctate Golgi
Manufacturer: Dako
Clone: 5-8A
CD99	Positive, diffuse	Positive, punctate Golgi
Manufacturer: Dako
Clone: MIC2
Myogenin	Negative	Negative
Manufacturer: Dako
Clone: FSD
CK7	Positive	Predominantly negative, few focal punctate positive cells
Manufacturer: Dako
Clone: OV-TL-12/30
CK20	Focal positive	Negative
Manufacturer: Dako
Clone: Kg20-8
TTF-1	Negative	Negative
Manufacturer: Leica
Clone: SPT24
CK5/6	Positive, membranous/cytoplasm	Negative
Manufacturer: Dako
Clone: D5/16 B4
Napsin	Negative	Negative
Manufacturer: Leica
Clone: 1P64
Synaptophysin	Negative	Negative
Manufacturer: Dako
Clone: DAK-Synap
Chromogranin	Negative	Negative
Manufacturer: Dako
Clone: DAK-A3
LCA (CD45)	Negative	Negative
Manufacturer: Dako
Clone: 2B11 P07/26
D2-40	Positive	Negative
Manufacturer: Dako
Calretinin	Positive	Negative
Manufacturer: Dako
Clone: Dako-Calret1
CK (cocktail)	Positive, membranous	Positive, punctate
Manufacturer: Dako and Life technologies
Clone: AE1/AE3 and Mak-6
A103	Negative	Negative
Manufacturer: Dako
S100	Negative	Focal positive
Manufacturer: Dako
Desmin	Negative	Negative
Manufacturer: Dako
Clone: D33
BCL2	Negative	Weak positive
Manufacturer: Dako
Clone: 124
CD15	Negative	Positive, focal punctate
Manufacturer: Dako
Clone: Carb3
PLAP	Negative	Negative
Manufacturer: Dako
Clone: 8A9
HMB45	Negative	Negative
Manufacturer: Dako
CD200	Positive	Positive
Manufacturer: R&D systems
FLI1	Positive, patchy nuclear	Positive, nuclear
Manufacturer: BD Bioscience
Clone: G146-222
WT1	Positive, weak nuclear and focal cytoplasmic	Positive, weak nuclear and focal cytoplasmic
Manufacturer: Biocare Medical
Clone Number: 6F-H2