Renal Tubular Dysgenesis in a Case of Fetus Acardius Amorphus

Figure 4

(a) PAS staining and immunohistochemistry staining for villin of fetal renal tissue. In immunohistochemistry, villin is stained in red (ALEXA 568) and nuclei in blue (Hoechst). Fetus acardius shows differentiated kidney with cortex and medulla, but lack of proximal tubules as shown by negative villin staining. Additionally, distal tubules of the fetus acardius amorphus show mild nephrocalcinosis (white star). PT (proximal tubule), G (glomerulus). Images are shown in 10x, 20x and 40x magnifications. (b) Hematoxylin and Eosin (HE) staining and immunohistochemistry staining for MAC 387 staining in fetal renal tissue. MAC 387 positive cells were detected in the interstitial tissue of the kidney in the Fetus acardius amorphus, those cells are marked with a black arrow in the Hematoxylin and Eosin (HE) staining labeling erythrocyte precursor cells. MAC 387 is stained in red (ALEXA 568), nuclei stained with in blue (Hoechst). Images are shown in 20x magnification. (c) CD31 immunohistochemistry staining in fetal renal tissue. CD31 (brown) is staining vessels in the renal cortex and medulla, nuclei are stained with hematoxylin in blue. Increased number of CD31 positive vessels is detected, in particular in the renal cortex of the fetus acardius amorphus.Images are shown in 10x magnification. (d) Smooth muscle actin (SMA) staining in fetal renal tissue. Increased number of SMA positive vessels in particular the cortex of the fetus acardius amorphus. SMA is stained in green, nuclei stained with Hoechst in blue. Glomeruli are marked with a white star, tubules with white arrows. Images are shown in 10x magnification.

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