Neurotrophic Receptor Tyrosine Kinase 2 (NTRK2) Alterations in Low-Grade Gliomas: Report of a Novel Gene Fusion Partner in a Pilocytic Astrocytoma and Review of the Literature

Figure 2

Pilocytic astrocytoma PML-NTRK2 gene fusion. (a) Illustrations of the breakpoints in NTRK2 on chromosome 9 and PML on chromosome 15, and associated transcripts, as determined by DNA next-generation sequencing (NGS). (b) Illustration of in-frame PML-NTRK2 gene fusion product confirmed with FusionPlex RNA NGS. (c) RT-PCR analysis validating PML-NTRK2 in-frame fusions F1 and F2. F1 = fusion transcript 1 with exon 3 of PML (5 end partner) and exon 16 of NTRK2 (3 end partner); F2 = fusion transcript 2 with exon 3 of PML (5 end partner) and exon 15 of NTRK2 (3 end partner). There are no detectable reciprocal 5-NTRK2-PML-3 fusion products.

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