Case Report
Cutaneous Coinfection of Cytomegalovirus and Mycobacterium chelonae Accelerated by Immunosuppression
Table 1
Antibodies used in the present study.
| | Target antigen | Animal/clone | Dilution | Antigen retrieval | Source |
| | Bacillus Calmette-Guérin (BCG) | Rabbit antiserum | 1 : 10,000 | None | Agilent | | MPT64 (RV1980C, 24 kDa protein) | Rabbit antiserum | 1 : 800 | HIER in 10 mM CB (pH 6) | Abcam | | LAM (lipoarabinomannan) | Mouse TMDU3 | 1 : 1,000 | HIER in 10 mM CB (pH 6) | MBL | | PAB (Propionibacterium acnes-specific lipoteichoic acid) | Mouse TMDU2 | 1 : 4,000 | HIER in 10 mM CB (pH 6) | MBL | | Bacillus cereus | Rabbit antiserum | 1 : 500 | HIER in 10 mM CB (pH 6) | Abcam | | Treponema pallidum | Rabbit antiserum | 1 : 1,000 | HIER in 1 mM EDTA (pH 8) | BM | | Escherichia coli | Rabbit antiserum | 1 : 20,000 | Proteinase K digestion | Agilent |
|
|
MPT: mycobacterial protein tuberculosis; HIER: heat-induced epitope retrieval; CB: citrate buffer; EDTA: ethylenediamine tetraacetic acid; Agilent: Agilent Technologies (Santa Clara, CA, USA); MBL: Medical and Biological Laboratories (Nagoya, Japan); Abcam: Abcam plc (Cambridge, UK); BM: Biocare Medical LLC (Pacheco, Philippines).
|
