Actin Colocalization with Metaphase Chromosomes of the Second Meiosis in Ovulated Mouse Oocytes
Colocalization of actin, labeled by antibody against fragment of C-terminal domain with metaphase chromosomes. (a–c) Colocalization of intact metaphase plate with actin; (a) fluorescent staining with antibody; (b) DNA staining by TO-PRO-3; (c) merged image, bar −20 μm. (d–f) Colocalization of single chromosomes in destructed metaphase plate of squashed oocytes with actin: (d) actin staining with antibody; (e) DNA staining by TO-PRO-3; (f) merged image; arrow—centromere region where colocalization is absent, bar −8 μm. (g–i) After latrunculin B treatment, the colocalization of actin with chromosomes was preserved; (g) fluorescent staining with antibody; (h) DNA staining by TO-PRO-3; (i) merged image, bar −10 μm.