Cooperation of Nectin-1 and Nectin-3 Is Required for Maintenance of Epidermal Stratification and Proper Hair Shaft Formation in the Mouse

Figure 4

Junctional defects of the compound mutant epidermis. Immunohistochemical analysis of dorsal epidermis of control ((a)–(c)) and nectin-1−/−; nectin-3+/− ((d)–(f)) at P10. E-cadherin is significantly reduced throughout the epidermis and ZO-1 is reduced in granular layer in the nectin-1−/−; nectin-3+/− epidermis ((a), (b), (d), and (e)). The membrane localization of desmoglein 1/2 is lost in the nectin-1−/−; nectin-3+/− ((c) and (f)). Dashed lines: basement membrane. Scale bar: 10 μm in (a)–(f).