Table of Contents
Dataset Papers in Biology
Volume 2013 (2013), Article ID 219095, 7 pages
http://dx.doi.org/10.7167/2013/219095
Dataset Paper

Adaptation of Escherichia coli ATCC 8739 to 11% NaCl

1School of Chemical and Life Sciences, Singapore Polytechnic, 139651, Singapore
2Department of Zoology, The University of Melbourne, Melbourne, VIC 3010, Australia

Received 2 July 2012; Accepted 8 August 2012

Academic Editors: A. Y. Çoban and K.-S. Yeh

Copyright © 2013 Jian Ann How et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Escherichia coli (E. coli) is a nonhalophilic microbe and used to indicate faecal contamination. Salt (sodium chloride, NaCl) is a common food additive and is used in preservatives to encounter microbial growth. The effect of how E. coli interacts with the salt present in the human diet is unclear. Thus, it is important to investigate this relationship. In order to adapt and survive the changes in the environment, E. coli may undergo halophilization. In this study, we observed the genetic changes and growth kinetics of E. coli ATCC 8739 under 3%–11% NaCl over 80 passages. Our results suggest that E. coli adapted to 1% increase in NaCl every month with a successful adaptation to 11% NaCl. Gram staining and PCR/RFLP showed that the cultures are Gram negative and the DNA profiles of all 4 replicates to be similar, suggesting that the cultures had not been contaminated.