Alterations of Hormone-Sensitive Adenylyl Cyclase System in the Tissues of Rats with Long-Term Streptozotocin Diabetes and the Influence of Intranasal Insulin

Table 2

The basal AC activity in plasma membrane fractions isolated from the heart, brain, and testes of insulin-treated and -untreated diabetic and control rats (in pmol cAMP/min per mg of membrane protein). C: control animals; CI: control animals treated with intranasal insulin (I-I); D: diabetic animals with 210-day streptozotocin-induced diabetes mellitus of the type 1 (T1DM); DI: diabetic animals treated with I-I.

Groups of Rats	Heart	Brain	Testes
Group C (n = 8)	30.4 ± 0.3; (C-D, P = 0.00000147; C-CI, P = 0.1433)	26.8 ± 0.5; (C-D, P = 0.0056; C-CI, P = 0.3619)	20.0 ± 0.2; (C-D, P = 10^−13; C-CI, P = 0.1685)
Average of the first series	30.5	29.0	18.1
Average of the second series	26.6	28.8	18.8
Average of the third series	27.3	25.6	21.0
Average of the fourth series	31.0	25.3	18.2
Group CI (n = 7)	28.9 ± 0.3; (CI-DI, P = 0.0003)	23.8 ± 0.7; (CI-DI, P = 0.7917)	18.2 ± 0.2; (CI-DI, P = 10^−7)
Average of the first series	31.1	25.7	16.0
Average of the second series	32.0	23.4	19.4
Average of the third series	26.3	22.1	19.5
Average of the fourth series	32.2	32.2	17.8
Group D (n = 8)	23.9 ± 0.5; (D-DI, P = 0.0012)	25.1 ± 0.2; (D-DI, P = 0.2915)	11.1 ± 0.3; (D-DI, P = 0.0001)
Average of the first series	21.5	25.8	10.1
Average of the second series	24.8	25.5	11.4
Average of the third series	24.2	24.7	10.6
Average of the fourth series	21.6	24.6	12.4
Group DI (n = 7)	26.8 ± 0.2	25.5 ± 1.3	12.4 ± 0.2
Average of the first series	28.9	23.9	11.5
Average of the second series	25.3	29.2	13.7
Average of the third series	24.4	25.1	13.9
Average of the fourth series	26.0	27.0	14.6

Values are expressed as the weighted mean ± weighted standard deviation of four individual experiments.