Alterations of Hormone-Sensitive Adenylyl Cyclase System in the Tissues of Rats with Long-Term Streptozotocin Diabetes and the Influence of Intranasal Insulin

Table 3

The stimulating effects of GppNHp (a) and forskolin (b) on the basal AC activity in the heart, brain, and testes in diabetic and control rats treated with I-I or saline. C: control animals; CI: control animals treated with intranasal insulin (I-I); D: diabetic animals with 210-day streptozotocin-induced diabetes mellitus of the type 1 (T1DM); DI: diabetic animals treated with I-I. GppNHp and forskolin were taken at 10⁻⁵ M. Values are the weighted mean ± weighted standard deviation of three individual experiments, each performed in triplicate.

(a)  

Groups of Rats	Heart	Brain	Testes
Group C (n = 8)	69.6 ± 1.7; (C-D, P = 10^−9; C-CI, P = 0.3460)	54.6 ± 0.6; (C-D, P = 0.0322; C-CI, P = 0.2613)	20.9 ± 0.3; (C-D, P = 10^−6; C-CI, P = 0.3268)
Average of the first series	68.0	63.5	20.1
Average of the second series	67.8	54.3	26.4
Average of the third series	74.7	52.6	23.8
Group CI (n = 7)	66.8 ± 1.0; (CI-DI, P = 0.0040)	54.0 ± 2.4; (CI-DI, P = 0.2172)	24.4 ± 1.0; (CI-DI, P = 10^−10)
Average of the first series	75.0	59.8	23.8
Average of the second series	63.1	45.4	24.9
Average of the third series	64.6	54.2	25.4
Group D (n = 8)	43.2 ± 1.1; (D-DI, P = 10^−7)	50.6 ± 1.5; (D-DI, P = 0.4860)	9.0 ± 0.2; (D-DI, P = 0.3766)
Average of the first series	42.0	56.0	9.0
Average of the second series	49.1	47.7	8.6
Average of the third series	42.4	48.9	9.8
Group DI (n = 7)	59.7 ± 1.2	47.5 ± 2.0	8.5 ± 0.3
Average of the first series	56.7	53.3	8.5
Average of the second series	57.5	41.9	9.6
Average of the third series	62.1	51.9	8.3

(b)  

Groups of Rats	Heart	Brain	Testes
Group C (n = 8)	201 ± 4; (C-D, P = 10^−8; C-CI, P = 0.1781)	166 ± 4; (C-D, P = 0.3585; C-CI, P = 0.2458)	41 ± 2; (C-D, P = 10^−9; C-CI, P = 0.3261)
Average of the first series	191	145	40
Average of the second series	204	173	42
Average of the third series	212	156	46
Group CI (n = 7)	203 ± 7; (CI-DI, P = 0.0211)	164 ± 3; (CI-DI, P = 0.0033)	38 ± 2; (CI-DI, P = 10^−6)
Average of the first series	173	178	44
Average of the second series	189	159	35
Average of the third series	209	158	41
Group D (n = 8)	145 ± 3; (D-DI, P = 0.0004)	152 ± 7; (D-DI, P = 0.5864)	18 ± 1; (D-DI, P = 0.2506)
Average of the first series	131	175	14
Average of the second series	155	138	23
Average of the third series	153	136	21
Group DI (n = 7)	166 ± 2	153 ± 3	23 ± 2
Average of the first series	171	161	27
Average of the second series	176	139	21
Average of the third series	162	135	18