Table of Contents
Dataset Papers in Science
Volume 2014 (2014), Article ID 105312, 4 pages
http://dx.doi.org/10.1155/2014/105312
Dataset Paper

The Equine CD4+ Lymphocyte Proteome

1Institute for Animal Physiology, Department of Veterinary Sciences, Ludwig Maximilians University Munich, Veterinärstraße 13, 80539 Munich, Germany
2Research Unit Protein Sciences, Helmholtz Center Munich, German Research Center for Environmental Health GmBH, Ingolstaedter Landstraße 1, 85764 Neuherberg, Germany
3Center for Ophthalmology, Institute for Ophthalmic Research, Eberhard Karls University of Tübingen, Röntgenweg 11, 72076 Tübingen, Germany

Received 1 April 2014; Accepted 27 July 2014; Published 4 September 2014

Academic Editor: Remond Fijneman

This dataset has been dedicated to the public domain using the CC0 waiver.

Dataset http://dx.doi.org/10.1155/2014/105312/dataset

Dataset

Dataset Item 1 (Table). Proteins identified from whole CD4+ cell lysate. Using mass spectrometry analysis (LC-MSMS), a total of 1250 different proteins could be identified from the whole CD4+ cell lysate. The column Accession Number shows the accession number of each identified protein and the column Protein Name shows the matching protein name as listed in Ensembl Horse protein database (http://www.ensembl.org/). If proteins were listed as “uncharacterized” in Ensembl Horse protein database, protein names were retrieved using protein Basic Local Alignment Search Tool (BLAST) from NCBI database (http://www.ncbi.nlm.nih.gov/). The column Peptide Count shows the amount of peptides used for the identification of each protein. The column Total Spectral Counts contains the spectral counts and Mean Normalized Abundance contains the mean normalized abundance of each identified protein from all six specimens used in the experiment. Confidence score >30 was required for a protein to be considered as identified in Ensembl Horse protein database and is shown in the column Confidence Score.

  • Column 1: Accession Number
  • Column 2: Protein Name
  • Column 3: Peptide Count
  • Column 4: Total Spectral Counts
  • Column 5: Mean Normalized Abundance
  • Column 6: Confidence Score

Dataset Item 2 (Table). Proteins identified from membrane fraction of CD4+ cells. Using mass spectrometry analysis (LC-MSMS), 376 different proteins were identified from the CD4+ membrane fraction. The column Accession Number shows the accession number of each identified protein and the column Protein Name shows the matching protein name as listed in Ensembl Horse protein database (http://www.ensembl.org/). If proteins were listed as “uncharacterized” in Ensembl Horse protein database, protein names were retrieved using protein Basic Local Alignment Search Tool (BLAST) from NCBI database (http://www.ncbi.nlm.nih.gov/). The column Peptide Count shows the amount of peptides used for the identification of each protein. The column Total Spectral Counts contains the spectral counts and Mean Normalized Abundance contains the mean normalized abundance of each identified protein from all six specimens used in the experiment. Confidence score >30 was required for a protein to be considered as identified in Ensembl Horse protein database and is shown in the column Confidence Score.

  • Column 1: Accession Number
  • Column 2: Protein Name
  • Column 3: Peptide Count
  • Column 4: Total Spectral Counts
  • Column 5: Mean Normalized Abundance
  • Column 6: Confidence Score