Role of Royal Jelly Treated Adipose-Derived Stem Cell-Extracellular Vesicles on Fibroblast Proliferation, Migration, and Collagen Production

Figure 6

Changes in miRNA-205-5p expression and sorting levels in ADSCs and EVs due to RJ treatment and its effect on EV-induced collagen synthesis in miR-205-5p antisense inhibitor- (Anti-miR-205-5p-) transfected NHDF. (a) Changes of miRNA 205-5p expression (internalization or sorting) levels in ADSCs and prepared EVs treated with RJ. Following each RJ treatment for 72 h, total RNA was extracted from each cell and EVs by TRIzol containing phenol/guanidinium isothiocyanate with DNase I treatment. Expression (internalization) levels of miRNA 205-5p in each cells and EVs were analyzed via a TaqMan® miRNA reverse transcription and TaqMan® miRNA assay kit using the real-time polymerase chain reaction. Relative rations are shown with the values of non-treated ADSCs (designated as 1). Data are presented as means ± SE (n = 6). Statistical analysis was performed using the Tukey–Kramer test, and differences were considered significant at . (b) Effects of Anti-miRNA-205-5p on intracellular collagen synthesis in nRJ-ADSC-EVs and pRJ-ADSC-EVs stimulated NHDFs. The cells transfected with Anti-miR-205-5p significantly suppressed collagen synthesis following nRJ-ADSC-EVs or pRJ-ADSC-EVs stimulation. Relative ratios are shown with the values of non-treated ADSCs (designated as 1). Data are presented as means ± SE (n = 9). Statistical analysis was performed using Student’s t-test, and differences were considered as statistically significant at .

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