Biochemical Characterization of a Thiol-Activated, Oxidation Stable Keratinase from Bacillus pumilus KS12

Table 3

Stability of the keratinase in the presence of various surfactants and detergents.

Surfactants/ Detergents (1% w/v, v/v)	Relative activity (%)
Control∗	100.00
Saponin	191.00
Sodium cholate	187.00
SDS	145.00
Triton-X	202.00
Tween-80	140.00
Detergents
Ariel	183.00
Henko	185.00
Fena	180.00
Nirma	183.00
Rin	180.00
Surf	160.00
Tide	179.00
Wheel	183.00

∗Control set up with inactivated enzyme was taken as 100% activity corresponded to 720 U/mL on feather keratin as substrate.