Purification, Characterization, and Effect of Thiol Compounds on Activity of the Erwinia carotovora L-Asparaginase

Table 4

Summary of activation parameters and affinity constants of thiol containing compounds to asparaginase.

Thiol compounds	𝛼	𝛽	K𝐴	K𝑚	Vmax	Vmax/K𝑚
			(mM)	(mM)	(𝜇moles mg^-1min^-1)
control	—	—	—	0.098	1666.7	17,007
Cys	0.589	1.87	293	0.086	2000	23,255
Met	0.138	1.96	229	0.076	2223	29,250
NAC	0.081	2.27	128	0.062	2380	38,387
GSH	0.062	1.89	14.6	0.055	2500	45,454