Production, Purification, and Characterization of Polygalacturonase from Mucor circinelloides ITCC 6025

Table 3

Purification of polygalacturonase produced extracellularly by Mucor circinelloides.

Step	Volume (mL)	Total Activity (IU)	Total Protein (mg)	Specific Activity (IU/mg)	Fold Purification	% Yield
Crude	1000	624	261	2.391	1	100
Ethanol (60%)	75	39.6	7.8	5.077	2.12	6.35
Sephacryl S-100	42	21.33	0.672	31.74	13.3	3.4