Research Article
Kinetics of Methylation by EcoP1I DNA Methyltransferase
Table 1
Primers for amplifying M.EcoP1I gene and oligonucleotides as DNA substrates used.
| | Oligonucleotides | |
| | CGCGGATCCGATGAAAAAAGAAACGATTTTTTCCGAAGTAG | Forward primer | | CGCGGATCCTTAGTTCCTTACCACTAAATC | Reverse primer |
| | Bt-TAGGTCAGAATTCAGCAGACCCTAAGTAGCC | Duplex I | | ATCCAGTCTTAAGTCGTCTGGGATTCATCGG |
| | Bt-CGATGCGACAGCAGACCTCTAGTCCAGCGAGACAGCAGACCTCTAGAGTCC | Duplex II | | GCTACGCTGTCGTCTGGAGATCAGGTCGCTCTGTCGTCTGGAGATCTCAGG |
| | CGATGCGACAGCAGACCTCTAGTCCAGCGAGACAGCAGACCTCTAGAGTCC | Duplex III | | GCTACGCTGTCGTCTGGAGATCAGGTCGCTCTGTCGTCTGGAGATCTCAGG |
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AGACC is EcoP1I recognition sequence.
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