Kinetics of Methylation by EcoP1I DNA Methyltransferase

Table 2

Kinetic parameters of EcoP1I DNA MTase.

DNA substrate	Mg^2+		Ca^2+		Mn^2+
	𝐾𝑚 (𝜇M)	𝑘cat (min^−1)	𝐾𝑚 (𝜇M)	𝑘cat (min^−1)	𝐾𝑚 (𝜇M)	𝑘cat (min^−1)
M13 dsDNA	0.42	0.0091	0.25	0.0084	0.15	0.0016
M13 ssDNA	0.31	0.0055	0.29	0.0046	0.14	0.0008
pUC19 DNA	0.31	0.0058	0.45	0.0050	0.21	0.0004
31mer duplex DNA	0.27	0.0066	0.38	0.0058	ND*	ND*

* ND: Not determined.