Screening of Bacterial Strains for Polygalacturonase Activity: Its Production by Bacillus sphaericus (MTCC 7542)

Table 4

Effect of various nitrogen sources on PGase production from Bacillus sphaericus.

Nitrogen source	Enzyme activity (𝜇mol min^-1 mL^-1)	Relative activity (%)
(NH4)2SO4	4.0±1.2	62.6
(NH4)NO3	5.1±0.3	80.2
NH4Cl	6.2±0.6	95.9
(NH4)2SO4⋅7H2O	1.2±0.9	19.5
Ca(NO3)2	1.8±0.3	28.3
KNO3	2.4±0.7	37.6
NaNO3	2.6±0.5	40.7
(NH4)H2PO4	3.4±0.2	52.9
Urea	1.7±0.6	26.9
Casein hydrolysate	5.0±0.9	78.6
YE	6.0±0.6	93.3
CH+YE (control)	6.4±0.8	100.0

Values are mean ± S.D. of 3 replicates.