Enzyme Inhibition by Molluscicidal Components of Myristica fragrans Houtt. in the Nervous Tissue of Snail Lymnaea acuminata
Table 2
In vitro effect of different concentrations (mM) of trimyristin and myristicin on the acetylcholinesterase (AChE), acid phosphatase (ACP), and alkaline phosphatase (ALP) activities in the nervous tissue of Lymnaea acuminata.
Treatment
Enzyme activity
Control
0.04 mM
0.07 mM
0.09 mM
0.1 mM
0.15 mM
IC50 mM
AChE
Trimyristin
0.72 ± 0.0 (100)
0.60 ± 0.0* (83)
0.52 ± 0.0* (72)
0.45 ± 0.0* (63)
0.38 ± 0.0* (53)
0.18 ± 0.0* (25)
0.11
Control
0.01 mM
0.02 mM
0.03 mM
0.05 mM
0.09 mM
Myristicin
0.72 ± 0.0 (100)
0.49 ± 0.0* (68)
0.42 ± 0.0* (58)
0.36 ± 0.0* (50)
0.29 ± 0.0* (40)
0.11 ± 0.0* (15)
0.03
Control
0.04 mM
0.07 mM
0.09 mM
0.1 mM
0.15 mM
ACP
Trimyristin
39.7 ± 0.1 (100)
35.5 ± 0.1* (90)
32.2 ± 0.0* (81)
27.9 ± 0.0* (70)
25.2 ± 0.1* (64)
8.1 ± 0.1* (20)
0.16
Control
0.01 mM
0.02 mM
0.03 mM
0.05 mM
0.09 mM
Myristicin
39.7 ± 0.1 (100)
33.6 ± 0.2* (85)
29.0 ± 0.0* (73)
25.8 ± 0.0* (65)
22.7 ± 0.0* (57)
7.2 ± 0.2* (18)
0.07
Control
0.04 mM
0.07 mM
0.09 mM
0.1 mM
0.15 mM
ALP
Trimyristin
35.0 ± 0.2 (100)
33.0 ± 0.0* (94)
29.7 ± 0.2* (85)
26.7 ± 0.3* (76)
23.2 ± 0.4* (66)
5.6 ± 0.2* (16)
0.18
Control
0.01 mM
0.02 mM
0.03 mM
0.05 mM
0.09 mM
Myristicin
35.0 ± 0.2 (100)
28.3 ± 0.0* (81)
25.1 ± 0.2* (72)
21.2 ± 0.3* (61)
18.9 ± 0.0* (54)
6.2 ± 0.1* (17.7)
0.06
Values are mean ±SE of six replicates. Values in parentheses indicate per cent of enzyme activity with control taken as 100%. Concentrations (mM) have been expressed as final concentration in the incubation mixture present in the cuvette. Acetylcholinesterase activity, mole “SH” hydrolyzed min-1 mg-1 protein. Acid phosphatase activity, mole substrate hydrolyzed 30 min-1 mg-1 protein. Alkaline phosphatase activity, mole substrate hydrolyzed 30 min-1 mg-1 protein.
*Significant (P < .05) when t-test was applied between treated and control groups.
