Research Article
Enzyme Inhibition by Molluscicidal Components of Myristica fragrans Houtt. in the Nervous Tissue of Snail Lymnaea acuminata
Table 3
Kinetic constants (
and
) of different enzyme inhibitions by trimyristin (0.09 mM) and myristicin (0.03 mM) in snail
Lymnaea acuminata.
| (a) Michaelis-Menten constant and of different enzymes were calculated from Lineweaver-Burk Plots (1/V versus 1/S). |
| | Enzymes | Untreated (Control) | Treated (Trimyristin) | Treated (Myristicin) |
| | | | | | | | | AChE | 3.1 × 10-4 | 1.2 | 5.5 × 10-4 | 1.0 | 3.8 × 10-4 | 0.95 | | ACP | 1.3 × 10-5 | 50.0 | 1.0 × 10-5 | 38.4 | 2.1 × 10-5 | 50.00 | | ALP | 2.0 × 10-5 | 62.5 | 2.2 × 10-5 | 55.5 | 1.6 × 10-5 | 50.00 |
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| (b) Michaelis-Menten constant and of different enzymes were calculated from nonlinear regression (V versus S). |
| | Enzymes | Untreated (Control) | Treated (Trimyristin) | Treated (Myristicin) |
| | | | | | | | | AChE | 2.9 × 10-4 | 1.1 | 4.9 × 10-4 | 0.84 | 3.2 × 10-4 | 0.80 | | ACP | 0.9 × 10-5 | 40 | 0.8 × 10-5 | 33 | 1.9 × 10-5 | 46 | | ALP | 1.8 × 10-5 | 56 | 1.3 × 10-5 | 45 | 1.3 × 10-5 | 47 |
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