Identification, Cloning, and Characterization of l-Phenylserine Dehydrogenase from Pseudomonas syringae NK-15
Figure 3
(a) Alignment of the amino acid sequence of D-phenylserine dehydrogenase (D-PSDH) with the sequences of 3-hydroxyisobutyrate dehydrogenase (TTHA0237) from Thermus thermophilus HB8 (PDB ID: 2CVZ) and 3-hydroxyisobutyrate dehydrogenase (PA0743) from Pseudomonas aeruginosa PAO1 (PDB ID: 3CUM). Conserved residues are marked with an asterisk and the key conserved catalytic residue is highlighted with a black background. The GXGXXG sequence fingerprint motif found in the Rossmann fold is boxed. (b) Alignment of the N-terminal amino-acid sequence of D-PSDH with the sequences of 6-phosphogluconate dehydrogenases from Ovis aries (PDB ID: 1PGN), Saccharomyces cerevisiae (PDB ID: 2P4Q), Lactococcus lactis (PDB ID: 2IYO), and Trypanosoma brucei (PDB ID: 1PGJ). Conserved residues are marked by an asterisk. The GX(G/A)XXG sequence fingerprint motif involved in coenzyme binding is boxed. The residues interacting with the -phosphate group of NADP+ are highlighted with a black background. Accession numbers for the proteins used are as follows: D-PSDH, AB499092 (GenBank™); TTHA0237, Q5SLQ6 (TrEMBL); PA0743, Q9I5I6 (TrEMBL); and 6-phosphogluconate dehydrogenase from O. aries, P00349 (Swiss-Prot); S. cerevisiae, P38720 (Swiss-Prot); L. lactis, P96789 (Swiss-Prot); T. brucei, P31072 (Swiss-Prot).
